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MERC Hyde Test Plan
12 April 2012
5
fresh municipal water, prior to conducting the subsequent trial. See SOPs for additional details
on test operations and sampling.
Table 2. MERC will be collecting a variety of data on physical, chemical, biological, and toxicological
parameters during this evaluation. In some case, values will be determined directly for the water using in
situ sensors or instruments. Table 2 describes samples collected and analyzed.
Viable Organisms >50µm in size:
The sampling system consists of two sets of paired canisters, each designed to
accommodate a 35 µm (50 µm diagonally) mesh plankton net used to collect the >50 µm size
fraction. One pair handles water from the treated ballast tank and the other pair handles water
from the control tank. The paired sampling canister/net arrangement allows for the residual from
the cod-end of one net from each pair to be processed for examination while filtration continues
via the other net, thereby avoiding clogging. In this way unimpaired filtration back and forth
between each pair of nets continues until a total of 3 to 7 m
3
has been processed from each of the
treated and control ballast tanks respectively. The sampling canisters are designed to allow
complete immersion of each net during the filtration process, thereby minimizing trauma to
filtered organisms. At the end of each trial (after three day hold times), the control and treated
ballast tanks are drained and processed as described above, with treated water first passing
through the Hyde system for additional UV irradiation (but not filtration) prior to sampling.
The proportion and total concentration of live versus dead organisms
> 50
µ
m
will be
determined using standard movement and response to stimuli techniques and this live/dead
analysis will take place within two hours of collecting the individual samples. Three m
3
is the
Parameter
Sample ID
Purpose
MERC Sample
Volume/Time points
Total Suspended Solids
(TSS) mg/L
Uptake Control
Quantify challenge
water
3-5 L at initial, mid and
final time points
Particulate Organic
Matter (as POC) mg/L
Uptake Control
Quantify challenge
water
3-5 L at initial, mid and
final time points
Dissolved Organic
Matter (as DOC) mg/L Uptake Control
Quantify challenge
water
3-5 L at initial, mid and
final time points
Chlorophyll-a µg/L
a. Uptake Control
b. Uptake Treated
c. Discharge Control
d. Discharge Treated
Quantify challenge
water
75 L time-integrated
sample with 2-L
subsamples
Viable Organisms > 50
µ
m / m
3
a. Uptake Control
b. Uptake Treated
c. Discharge Control
d. Discharge Treated
Quantify live
organisms > 50
µ
m
3-10 m
3
time-integrated
samples
Viable Organisms 10-
50
µ
m / ml
a. Uptake Control
b. Uptake Treated
c. Discharge Control
d. Discharge Treated
Quantify live
organisms 10 – 50
µ
m
75 L time-integrated
sample with 2-L
subsamples
Bacteria cfu/ml
a. Uptake Control
b. Uptake Treated
c. Discharge Control
d. Discharge Treated
Quantify regulated
indicator pathogens and
total heterotrophic
bacteria
75 L time-integrated
sample with 2-L
subsamples