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Appendix D
5/20/2009
7
6.
EQUIPMENT AND SUPPLIES
6.1 A fluorometer equipped with the proper light source and filters for
chlorophyll analysis. This laboratory uses a Turner Designs TD700
fluorometer equipped with a daylight white lamp, 340-500 nm excitation filter
and >665 nm emission filter, and a Turner Designs Trilogy fluorometer
equipped with either the non-acid or the acid optical module.
6.2 Freezer, capable of maintaining -20
° ±
5
°
C.
6.3 Lab ware – All reusable lab ware (glass, Teflon, plastic, etc) should be
sufficiently clean for the task objectives.
6.4 A centrifuge.
6.5 A Teflon pestle for grinding, either by hand or power, and/or a sonicator.
7.
REAGENTS AND STANDARDS
7.1 Purity of Water – Unless otherwise indicated, references to water shall be
understood to mean reagent water conforming to ASTM Specification D
1193, Type I. Freshly prepared water should be used for making the
standards intended for calibration. The detection limits of this method will
be limited by the purity of the water and reagents used to make the
standards.
7.2 Purity of Reagents – Reagent grade chemicals shall be used in all tests.
Unless otherwise indicated, it is intended that all reagents shall conform to
specifications of the Committee on Analytical Reagents of the American
Chemical Society, where such specifications are available. Other grades
may be used, provided it is first ascertained that the reagent is of
sufficiently high purity to permit its use without compromising the
accuracy of the determination.
7.3 Acetone (H
2
C=O=CH
2
), 90% v/v
Acetone, reagent grade
900 ml
De-ionized water
100 ml
Using a graduated cylinder, add 100 ml de-ionized water to 900 ml
acetone.
7.4 Hydrochloric Acid, 5 % (v/v) –
Hydrochloric acid (HCl), concentrated, 5.0 ml
De-ionized water, q.s.
100 ml
In a 100 ml volumetric flask, add 5.0 ml of concentrated hydrochloric
acid to ~60 ml of de-ionized water. Dilute to 100 ml with de-ionized
water.
7.5 Blanks – A reagent blank of 90% acetone is used.
7.6 Standards – Standards used are one of the following:
7.6.1 Turner Designs Fluorometer standards, PN 10-850. These include
a set of 2 ampoules, 1 high and 1 low, accompanied by certification from
Turner Designs.