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Appendix D
5/20/2009
15
11.6.2.4
To determine mg/g total sample:
11.6.3
Epiphyte Mylar strips:
11.6.3.1
Total chlα:
By dividing by the area of the strip, the units then become µg/m
2.
Where:R
B
= reading before adding acid
R
A
= reading after adding acid
40 = extract volume in milliliters
r = acid ratio: R
B
/R
A
F
S
= calibrating std/reading of std
12
References
:
12.1 Strickland, J.D.H. and T.R. Parsons. 1972. A practical handbook of
seawater analysis. Bulletin 167 (2
nd
Ed.). Fisheries Research Board of Canada,
Ottawa, Canada.
12.2 Parsons, T.R., Y. Maita and C.M. Lalli. 1984. Determination of
chlorophylls and total carotenoids: Spectrophotometric method. pp. 101 - 112
in
Parsons, T.R., Y. Maita and C.M. Lalli. A manual of chemical and biological
methods for seawater analysis. Pergamon Press, Oxford.
12.3 EPA Method 445.0: In Vitro Determination of Chlorophyll a and
Phaeophytin a in Marine and Freshwater Algae by Fluorescence.
12.4 Welschmeyer, N.A. 1994. Fluorometric analysis of chlorophyll
a
in the
presence of chlorophyll
b
and phaeopigments. Limnol. Oceanogr., 39: 1985-
1992.