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MERC+GSI – DRAFT Test Plan
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Table 5. Summary of Test Conditions and Test Acceptability Criteria for the Sheepshead
Minnow
Cyprinodon variegatus
Larval Survival and Growth Chronic Test
Test type:
Static renewal
Test Duration:
7 d
Temperature:
25 ºC (± 1ºC)
Lighting:
Normal laboratory fluorescent
Photoperiod:
16 h light, 8 h dark
Test chamber size:
500 ml
Test solution volume:
250 ml
Renewal of test solutions:
Daily
Age of test organisms:
Newly hatched larvae <24 hours old
No. larvae per test chamber:
10
No. replicate chambers per concentration:
4
No. larvae per test concentration:
40
Feeding regime:
Artemia
nauplii (<24 h old).
On days 0-2, feed
0.10g wet weight newly hatched (<24 hours
old) brine shrimp nauplii daily. On days 3-6,
feed 0.15g wet weight newly hatched (<24
hours old) brine shrimp nauplii daily.
Cleaning:
Siphon daily, immediately before test solution
renewal
Aeration:
None, unless DO concentration falls below 4.0
mg/l. Rate should not exceed 100 bubbles/min
Dilution water:
Baltimore Harbor water collected at the same
time as the initial untreated ballast water
Test concentrations:
100, 56, 32, 18, and 10 % ballast discharge or
receiving water by volume plus a Baltimore
Harbor and a Wye River control
Dilution factor:
0.56
Endpoint:
Survival and growth (dry weight)
Test acceptability criteria:
80% or greater survival in controls; average
dry weight per surviving organism in control
chamber equals or exceeds 0.60 mg