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MERC+GSI – DRAFT Test Plan
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Table 7. Summary of Test Conditions and Test Acceptability Criteria for the Mysid
Americamysis bahia
Larval Survival and Growth Chronic Test
Test type:
Static renewal
Test Duration:
7 d
Temperature:
26ºC (± 1ºC)
Lighting:
Normal laboratory fluorescent
Photoperiod:
16 h light, 8 h dark
Test chamber size:
400 ml
Test solution volume:
150 ml
Renewal of test solutions:
Daily
Age of test organisms:
7 d
No. organisms per test chamber:
5
No. replicate chambers per concentration:
8
No. organisms per test concentration:
40
Feeding regime:
Feed 150 <24 h old
Artemia
nauplii daily, half
after test solution renewal and half after 8-12 h.
Cleaning:
Siphon daily immediately before test solution
renewal and feeding.
Aeration:
None, unless DO concentration falls below 4.0
mg/l. Rate should not exceed 100 bubbles/min
Dilution water:
Baltimore Harbor water collected at the same
time as the initial untreated ballast water
Test concentrations:
100, 56, 32, 18, and 10 % ballast discharge or
receiving water by volume plus a Baltimore
Harbor and a Wye River control
Dilution factor:
0.56 dilution series
Endpoint:
Survival and growth (dry weight)
Test acceptability criteria:
80% or greater survival in controls; average
dry weight per surviving organism in control
chamber equals or exceeds 0.20 mg; fecundity
may be used if 50% or more of females in
controls produce eggs.