Basic HTML Version
Appendix C
5/20/2009
13
min sparge. The instrument allows up to a 4
point curve.
11.1.8.3
TIC: This analysis is not performed on a
regular basis; therefore the samples are analyzed
using a curve of 0-30 ppm Na
2
CO
3
/NaHCO
3
and an injection volume of 25
µ
l in range 1. If
the samples fall within the low end of the curve,
the curve range and injection volume are
adjusted accordingly. If the samples fall off
scale, they are diluted.
11.1.9
Use the large sample vials for the curve stds. Examples of
sample protocol can be found in the data sheet notebook.
11.1.10 I always load several DHOH samples (at least 3) as
conditioning samples.
11.1.11 Fill std vials ~ 1/3 to 1/2 full.
11.1.12 Fill sample vials to within 3/4 - 1" of the top. Acidify the
NPOC samples by adding 100 µl of 2N HCl. The
instrument can be set up to automatically add acid to the
standard cups. Make sure a standard cup filled with 2N
HCl is in position S8. DO NOT acidify TIC samples.
11.1.13 Data sheets are found in the LOTUS TOC5000 folder,
labeled TOC datasheet, high, low std. Print as many as
needed. (Low std set to print 5; high std prints 2, and plain
datasheet prints 1.) Examples are found before section 12.
11.1.14 When the turntable is loaded, place in the ASI. Line up
pins to align properly. Put top on lining up pins carefully.
Sample needles will Z if the top is not properly aligned.
11.1.15 Go to the MAIN MENU — Press 9 and Enter for the
AUTOSAMPLER.
11.1.16 Determine analysis type: Press 1 for TC, 2 for IC, 3 for
TOC, 4 for NPOC on each to be used, one line per curve.
The instrument can store up to 18 curves.
11.1.17 Create 2 std curves for NPOC. Using the number keys,
toggle the sample type to NPOC. Enter sample positions
under IS and FS, and curve # under C1. Curve #1 is used
as the conditioning curve. At least vials 1 -3 of DHOH and
the std DHOH (S1). (9 max 10 injections, 100
µ
l, range =
1, 3 minute sparge, and curve not through zero.) Curves 2-
18 are used as sample curves. See 11.1.19.
11.1.18 If analyzing TIC, create 1 std curve for TIC. Using the
number keys, toggle the sample type to TIC.
11.1.19 Enter curve # under C1 and ENTER. This opens the
screen to input curve data. Use arrow keys to navigate.
Enter std conc. & position (S1, S2, etc.)