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Appendix C
5/20/2009
15
11.3.6
The furnace automatically turns on when connected. It takes
20-30 minutes to come to temperature.
11.3.7
To view instrument conditions, click on View /Background
Monitor. When all conditions are OK, the system is ready to
run.
11.3.8
Loading Samples: Decide on standard curve. I use 2 curves:
Low — 0-10 ppm ---- DHOH, 2.0, 5.0, 10.0 as calibration
standards, and adding 0.5, 1.0, and 7.5 as well for stds as
samples. 60 µL injection. High-----
0-20 ppm ----- DHOH, 5.0, 10.0, 20.0 as calibrating STDs and
adding 1.0, 2.0, & 15.0 as STDs as samples. 30 µL
injection. The instrument allows up to a 4 point curve.
11.3.9
Use the large sample vials for the curve stds. Examples of
sample protocol can be found in the data sheet notebook.
11.3.10 I always load several DHOH samples (at least 3) as
conditioning samples
11.3.11 Fill std vials ~ 1/3 to 1/2 full.
11.3.12 Fill sample vials to within 3/4 - 1" of the top.
11.3.13 Data sheets are found in the LOTUS TOC5000 folder on the
desktop, labeled TOC datasheet, high, low std. Print as many
as needed. (Low STD set to print 5; high STD prints 2, and
plain datasheet prints 1.)
11.3.14 Acidify the samples by adding 100 µl of 2N HCl. The
standard curve files are set up to automatically add acid to the
standard cups. Make sure a standard cup filled with 2 N HCl
is in position S8.
11.3.15 When the turntable is loaded, place in the ASI. Line up pins
to align properly. Put top on lining up arrows carefully.
Sample needles will Z if the top is not properly aligned.
11.3.16 Close the Background Monitor and a blank sample table will
be opened.
11.3.17 Enter sample table by clicking on Edit/Insert Std. Click on
CONDBLK.CAL. Then click on Edit/Autogenerate. In the
method name slot, type CONDBLK.MET. Using the TAB,
toggle through to fill in the blanks. This is an NPOC file. I
usually put conditioning DHOH vials in positions 1-3. The
standard curve is next. Several curves are already stored.
Click on Edit/ insert STD again to choose the proper curve.
LOSTD10 is a 0-10 ppm curve with DHOH, 2.0, 5.0, & 10 in
positions S1, 2, 3, 4. HISTD20 is a 0-20 ppm curve with
DHOH, 5.0, 10.0, 20.0 in positions S1, 2, 3, 4.
11.3.18 Click on Edit/ Insert Sample next. Most likely this is a new
file, so click on NEW. You will then be prompted enter the
conditions of the samples to be run. Enter a file name (up to 8
characters), and TAB to the next slot. Enter the sample
information (ex. DNR Potomac 3/30/08). TAB again to enter