Basic HTML Version
14
Parameter Sub-category
G8
ETV
MERC
Water Quality
Measurements N/A
pH, temperature, salinity,
dissolved oxygen, TSS, DOC,
POC and turbidity (NTU)
should be measured at the same
time that the samples are
collected.
Temperature, salinity, TSS,
POM, DOM, mineral
matter, dissolved oxygen,
pH, chlorophyll a.
Dependent on test plan.
May include salinity, DOC,
POC; Mineral Matter; TSS,
dissolved oxygen;
temperature, pH, total
chlorophyll and others as
required.
Toxicity
N/A
Separate samples should be
collected for toxicity testing of
treated water, from the
discharge, for systems that
make use of Active Substances
and also for those, which could
reasonably be expected to
result in changes to the
chemical composition of the
treated water such that adverse
impacts to receiving waters
might occur upon discharge.
Tests should conducted in
accordance with paragraphs
5.2.3 to 5.2.7 of the Procedure
for Approval of Ballast Water
Management Systems That
Make Use of Active Substances
(resolution MEPC.126(53)) as
amended .
Toxicity tests will be
conducted for treatments
involving biocides. Tests
will be selected from a
short list of U.S. EPA
standard tests.
Dependent on test plan,
e.g., Whole effluent
toxicity (WET) tests and
residual byproduct
chemical analyses using
treatment discharge water
for systems involving
active substances.
Biological
Sample
Analysis
N/A
Samples should be analyzed as
soon as possible after sampling,
and analyzed live within 6 hour
or treated in such a way as to
ensure that proper analysis can
be performed. Widely accepted
standard methods for the
collection, handling, storage,
and analysis of samples should
be used.
Zooplankton
enumeration:
Concentrate using 35
µm mesh plankton nets;
no preservation; sub-
sample into well plate
(20 1mL wells
observed); observe with
dissecting microscope
and probe organisms to
determine live/dead
status; fix with Lugol’s
for total counts.
Phytoplankton
enumeration: No
preservation; stain with
Fluorescein Diacetate
(FDA) and CMFDA;
load into a Sedgewick
Rafter
Counting Chamber and
examine under
epifluorescence using a
FITC narrow pass filter
cube. Bacteria: Plate on
appropriate media; use a
DNA colony blot
hybridization for V.
cholerae.
Direct counts (number live)
for treatment discharge
samples, indirect counts
(number of dead and total)
for intake and control
discharge samples for
organisms in the >50 µm
size class; direct counts
(number of live) using
FDA+CMFDA vital stain
for organisms in the 10-50
mm size class; enumeration
(using appropriate media)
of total viable
heterotrophic bacteria,
E.
coli
, and Enterococci and
preparation of colony blots
for the detection of
toxigenic
Vibrio cholerae
.
Flow Rate
N/A
At least 200 m
3
/hr.
At least 200 m
3
/hr.
Up to 350 m
3
/hr and no
lower than 100 m
3
/hr.