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SOP10 – Toxicity Analysis
95
Summary of Test Conditions and Test Acceptability Criteria for the Algae
Isochrysis galbana
Chronic Growth Test
Test type:
Static non-renewal (required)
Temperature:
20 ºC ± 1 ºC
Light quality
“Cool white” fluorescent lighting
(recommended)
Light intensity:
360-440 foot candles
Photoperiod:
Continuous illumination
Test chamber size:
250 ml
Test solution volume:
100 ml
No. replicate chambers per
concentration:
4
Renewal of test solutions:
None
Age of test organisms:
Log growth phase
Initial cell density in test chambers:
1-2 X 10
4
cells ml
-1
Shaking rate:
100 rpm continuous on a mechanical
shaker or twice a day hand shaken
Aeration:
None
Nutrient solution
Algal assay culture medium nutrients
added to each replicate (Appendix A3 of
ASTM Designation E 1218-04; ASTM,
2006)
Dilution water:
Baltimore Harbor water collected at the
same time as the initial control ballast
water
Test concentrations:
100, 56, 32, 18, and 10 % ballast discharge
or receiving water by volume plus a
Baltimore Harbor and a Wye River control
Dilution factor:
0.56 dilution series
Test duration:
96 hours
Endpoint:
Growth (cell counts)
Test acceptability criterion:
Mean cell density of at least 1 x 10
6
cells
ml
-1
in the controls; and variability (CV%)
among control replicates less than or equal
to 20%