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SOP6 – Water Quality Analysis
42
7.3.7. End of Day Sample Transfer
After testing is complete for the day, place the labeled bag containing filters in an iced cooler for
transport back to CBL. Freeze or immediately transport to NASL. Sign the chain of custody
lines. Make a copy of the form for MERC files.
7.4. Chlorophyll 5-day Regrowth Experiment (optional)
Regrowth experiments are occasionally conducted on treated samples to look for measureable
Chl-a.
7.4.1. Materials for regrowth
Growth Chamber
Equipment
Filtering Equipment Light and Nutrient
Equipment
Other
Equipment
-
10 autoclaved 500
ml flasks*
-
Bio-shield
sterilization wrap
(blue)
-
Foil squares
-
Rubber bands
-
Small brass 125
micron sieve
-
Small funnel
-
2, 500 ml
autoclaved to-
deliver graduated
cylinders* (one for
treatment and one
for control)
-
2 filtering rigs for
Chl-a (one for
treatment and one
for control)
-
Light array
-
24 h timer
- Growth nutrients –
nitrate, phosphate,
and silica salt
-
200 µl pipette and
tips
-
0.2 micron filtered
seawater,
autoclaved
-
Gloves
-
Kim wipes
Autoclave 10 acid-washed flasks and 2, 500 ml cylinders (cover openings with the blue wrap,
then foil) at 121 degrees °C for 20 minutes with 15 minute cool-down.
7.4.2. Set-up for Regrowth Experiment
Wearing gloves, randomly choose a sample bottle from the cooler, shake well, sample rinse a
correctly labeled (“T” or “C”) 500 ml to-deliver graduated cylinder, then measure out 350 mls of
sample.
Using a small 125 µM sieve and a funnel, coarse-filter the 350 mls sample into a numbered flask.
Use filtered seawater to rinse the sieve and funnel. Re-cover the flask with its own blue wrap and
foil. Place each flask on the unlit “incubation light table”.
a.
Note: Use a Kim wipe to cover the to-deliver graduated cylinder opening when
not in use.
b.
On the data sheet, record the flask number next to the corresponding sample bottle
number.
c.
Note: Randomly place the flasks on the light table.