Basic HTML Version
SOP7 – Live Organisms >50 μm
55
split and subsample volumes. Other splits/beaker-volumes may be used if too few or too many
animals are present; however, a 1/2500 split tends to be a good place to start. Place subsample in
labyrinth sorting tray. Quantify all organisms noting all taxa present. Perform 3-replicates of this
assay.
When sample analysis is complete, sieve/rinse with tap water back into the same sample bottle.
Adjust volume in bottle to about 45 ml with tap water and fill with 400 ml full strength ethanol
to obtain a 10% ethanol concentration. Rinse all equipment that has been in contact with sample
with tap water prior to placing in storage or starting another sample.
8.4. QA/QC
Quality assurance and quality control of the sample count can be checked with the MicroCap
v3.0 (ESPA Systems CO, LTD. www.espa.com.tw) video capture software in real time using the
computer monitor or the video file reviewed afterward. Microscopes are calibrated once a year to
a NIST certified micrometer.
8.5. References
Omori and Ikeda 1984. Methods in Marine Zooplankton Ecology. John Wiley & sons, 332 pp.
UNESCO 1968. Zooplankton Sampling. Monographs on Oceanographic Methods. United
Nations Education, Science, and Cultural Organization. 137 pp.