Basic HTML Version
SOP8 – Live Organisms 10-50 μm
67
12.
To maintain consistency among sites and samples, chambers will only be analyzed for 20
min after incubation regardless of whether or not background fluorescence was seen at
the time.
Graphs will be generated comparing % Organisms (10-50um) vs 4 categories of Movement and
Fluorescence (M+/F+, M+/F-, M-/F+, M-/F-) for each treatment (live, dead-heated, dead-frozen)
9.10. References
Hasle, G.R. 1978. The inverted-microscope method. Chapter 5.2.1 in Phytoplankton Manual. A.
Sournia, ed. United Nations Educational, Scientific and Cultural Organization. Paris. 337
pp.
Lund, J.W. Kipling, G.C., and Le Cren, E. E., 1958. The inverted microscope method of
estimating algal numbers and the statistical basis of estimation by counting.
Modigh, M and Castaldo, S. (2005) Effects of fixatives on cilates as related to cell size. Journal
of Plankton Research,
27
(8), 845-849.
Paxinos, Rosemary and Mitchell, James G. (2000) A rapid Utermohl method for estimating
algal numbers. Journal of Plankton Research,
22
(12), 2255-2262.
Standard Operating Procedure for Plankton Analysis, LG401, Revision 03, February 2003.
Stoecker, D. K.
,
Gifford, D. J. and Putt, M. (
1994a
) Preservation of marine planktonic ciliates:
losses and cell shrinkage during fixation. Mar. Ecol. Prog. Ser.
,
110
,
293
–
299.
Steinburg, Mia K., Lemieux, Edward J. and Drake, Lisa A. (2011) Determining the viability of
marine protists using a combination of vital, fluorescent stains. Marine Biology,
158:
1431-
1437.
Tang, Ying Zhong and Dobbs, Fred C. (2007) Green Autofluorescence in Dinoflagellates,
Diatoms, and Other Microalgae and Its Implications for Vital Staining and Morphological
Studies.
Applied and Environmental Microbiology,
Apr. 2306-2313.