Basic HTML Version
SOP10 – Toxicity Analysis
79
Samples will be analyzed for TRO immediately upon collection onboard the mobile testing
platform
to avoid loss of oxidant due to holding. In addition to the amperometric titration method
we will use a YSI Multimeter (Model # 556) equipped with a probe to measure oxidation
reduction potential (ORP). The probe uses a platinum button sensor giving the instrument a
range of -999 to +999 mV, an accuracy of ± 20 mV and a Resolution of 0.1 mV.
11.4. Experimental Design and Test Conditions
Toxicity tests are conducted on the discharge from at least one test trials for all treatment systems
evaluated (if not chemical or biocide) and for ALL test trials if the treatment utilizes active
substances. The discharge water is tested with three estuarine species as described above.
Chronic data are generated for each test including the algae test, which represents a true
population growth test. A dilution series, using ambient water, is run for each species.
Test samples are collected at the time of discharge
via an integrated flow system into a toxicity-
designated, whole water reservoir, as described above and in section 4.5, 7.2.4 and 9.2.
Immediately after filling of toxicity reservoir,
20 L and 65 L samples, respectively, are collected.
This includes enough water to conduct all of the test renewals. Test water is stored in 20-l glass
containers and held at 4°C in the dark to retain as much of the initial toxicity as possible.
Standard EPA (2002) and ASTM (2006) methods to conduct Whole Effluent Toxicity tests and
single compound toxicity tests are employed. The survival and growth end-points from these
tests are those required by the G9 document in Section 5.2.4 (IMO, 2008). The algae test
represents a true population growth test.