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MERC BIO-SEA Test Plan
7 June 2012
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7. Data Analysis
As noted above, continuous time-integrated samples and measures from each tank will be
taken. Consequently please note that although certain assays employ replicates or sub-samples
during analysis, to avoid pseudo-replication, the unit of replication for statistical analyses is each
trial (n = 5 or 6). We assume that all measures for a single trial provide one estimate of
treatment efficacy. Thus, treatment efficacy for any biological parameter is estimated as changes
found before and after trial (percent reduction), and as the difference in concentration between
treated water and IMO standards. This approach controls for variation due to temporal changes
in environmental conditions.
Quality Assurance and Quality Control policies and procedures, data recording
processing and storage, and detailed roles and responsibilities can be found in the MERC QMP,
QAPP and SOPs.
8. Evaluation Schedule
(planned dates based on current plan and may vary):
•
MERC Test Plan for BIO-SEA finalized and Evaluation Agreement signed by June 15,
2012
•
Delivery and installation of BIO-SEA system, July 5 – 13, 2012
•
MERC evaluation of BIO-SEA system in Baltimore MD initiated by July 16, 2012
•
MERC will complete sample analysis and compile data from the evolution by November
2012
•
MERC will distribute a draft report on the performance of the BIO-SEA system for
review by the MERC Advisory Board and BIO-UV representatives by December 2012
•
MERC will submit a final report to MPA, MARAD and BIO-UV by January 2013
9. References
ASTM. 2006. Standard Guide for Conducting Static Toxicity Tests with Microalgae.
Designation E 1218-04. Annual Book of ASTM Standards Section Eleven Water and
Environmental Technology Volume 11.06 Biological Effects and Environmental Fate;
Biotechnology. ASTM International, West Conshohocken, PA.
Environmental Protection Agency. 2002. Guidelines establishing test procedures for the analysis
of pollutants; whole effluent toxicity test methods. 40 CFR Part 136. Rules and Regulations
Vol. 67, No. 223.
Environmental Protection Agency. 2002. Short-term Methods for Estimating the Chronic
Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms. 3
rd
Edition.
EPA-821-R-02-014.
Guillard, R. L. 1975. Culture of phytoplankton for feeding marine invertebrates, pp. 29-60. In:
Culture of Marine Invertebrate Animals. W. L. Smith and M. H. Changley, eds., Plenus
Publishing Corp., New York
Hasan, J. A., Huq, A., Tamplin, M. L., Siebeling, R. J. and R.R. Colwell. (1994). A novel kit for
rapid detection of
Vibrio cholerae
O1.
J Clin Microbiol
. 32: 249–252.
Miller, A.W., M. Frazier, G.E. Smith, E.S. Perry, G.M. Ruiz, and M.N. Tamburri, 2011.
Enumerating Sparse Organisms in Ships’ Ballast Water: Why Counting to 10 is Difficult?
Environ. Sci. Tech.
, 45:3539-3546.
Steinberg, M.K., E.J. Lemieux, and L.A. Drake, 2011. Determining the viability of marine
protists using a combination of vital, fluorescent stains.
Mar Biol
158:1431-1437.