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MERC Hyde Test Plan
12 April 2012
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required in Section 5.2.4 of the G9 document (IMO, 2008). Tests are designed with a dilution
series to allow calculation of daily LC50 (concentration yielding 50% lethality) values from
acute and chronic mortality data. In addition, chronic tests will include sufficient treatment
replication to allow calculation of NOEC (no observable effect concentration), LOEC (lowest
observable effect concentration) and EC25 (percent concentration yielding a 25% effect) values
for all toxicity endpoints as required in Section 5.2.5 of the G9 (IMO, 2008). Statistical analyses
will be performed using ToxCalc statistical software (TSS, 2006) according to methods from
USEPA (2002) and ASTM (2006) guidance documents. A test trial will be considered a failure
on the grounds of residual toxicity upon discharge if acute lethality (as indicated by
determination of an LC50 of less than 100%) occurs in any test species.
6. Test Trials
After one mechanical calibration run, and successful completion of the O&M verification
trial (with no more than one mechanical failure, described above), MERC will conduct a
maximum of six test trials (three for each filter option) of the treatment system to assess its
ability to meet IMO D2 standards in land-based testing during the spring of 2012. For any test
that is considered valid (and for which the facility testing system functioned properly and the
average discharge from the control water is greater or equal to 10 times D2), an inability to: (a)
successfully treat ballast water without interruption and/or (b) to meet D2 discharge standards
after a three-days holding time, will be considered a “failure”. Results of tests regarded as
failures will be noted and included in the final report. Two failures on the part of the treatment
system may result in the termination of testing prior to the maximum of six test trials depending
on the nature of the failures. MERC Senior Management will make a final decision on early
termination of the tests, in consultation with Hyde representatives.
This evaluation will be based on physical and biological characterization of water upon
ballasting (uptake of water) and comparisons of organisms in control versus treated water after a
three-day, in-tank holding time for the different D2 biological categories. Results will also be
presented as concentration of viable organisms per biological category in treated water upon
discharge versus D2 standards.
Table 3. A summary of the trials to be conducted, including target TSS levels. The order and specific
filters used in these trial may be modified based on availability and results of initial internal testing by
Hyde Marine.
Trail #
Treatment
Trial Type
Target TSS
1
Hyde 40
µ
m screen filter + UV
Calibration*
Ambient Water
2
Hyde 40
µ
m screen filter + UV
O&M
Ambient Water
3
Hyde 40
µ
m screen filter + UV
Biology
~ 25 mg/L
4
Hyde 40
µ
m screen filter + UV
Biology
~ 50 mg/L
5
Hyde 40
µ
m screen filter + UV
Biology
~ 75 mg/L
6
Hyde 55
µ
m disc filter + UV
Calibration*
Ambient Water
7
Hyde 55
µ
m disc filter + UV
O&M
Ambient Water
8
Hyde 55
µ
m disc filter + UV
Biology
~ 25 mg/L
9
Hyde 55
µ
m disc filter + UV
Biology
~ 50 mg/L
10
Hyde 55
µ
m disc filter + UV
Biology
~ 75 mg/L
* Data not reported.