Basic HTML Version
MERC Hyde Test Plan
12 April 2012
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7. Data Analysis
As noted above, continuous time-integrated samples and measures from each tank will be
taken. Consequently please note that although certain assays employ replicates or sub-samples
during analysis, to avoid pseudo-replication, the unit of replication for statistical analyses is each
trial (n = 5 or 6). We assume that all measures for a single trial provide one estimate of
treatment efficacy. Thus, treatment efficacy for any biological parameter is estimated as changes
found before and after trial (percent reduction), and as the difference in concentration between
treated water and IMO standards. This approach controls for variation due to temporal changes
in environmental conditions.
Quality Assurance and Quality Control policies and procedures, data recording
processing and storage, and detailed roles and responsibilities can be found in the MERC QMP,
QAPP and SOPs.
8. Evaluation Schedule
(planned dates based on current plan and may vary):
•
MERC Test Plan for Hyde finalized and Evaluation Agreement signed by March 2012
•
Delivery and installation of Hyde system, first week of April 2012
•
MERC evaluation of Hyde systems in Baltimore MD initiated by April 2012
•
MERC will complete sample analysis and compile data from the evolution by Oct 2012
•
MERC will distribute a draft report on the performance of the Hyde system for review by
the MERC Advisory Board and Hyde representatives by November 2012
•
MERC will submit a final report to MPA, MARAD and Hyde by December 2012
9. References
ASTM. 2006. Standard Guide for Conducting Static Toxicity Tests with
Microalgae. Designation E 1218-04. Annual Book of ASTM Standards Section Eleven
Water and Environmental Technology Volume 11.06 Biological Effects and Environmental
Fate; Biotechnology. ASTM International, West Conshohocken, PA.
Environmental Protection Agency. 2002. Guidelines establishing test procedures for the analysis
of pollutants; whole effluent toxicity test methods. 40 CFR Part 136. Rules and Regulations
Vol. 67, No. 223.
Environmental Protection Agency. 2002. Short-term Methods for Estimating the Chronic
Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms. 3
rd
Edition.
EPA-821-R-02-014.
Guillard, R. L. 1975. Culture of phytoplankton for feeding marine invertebrates, pp. 29-60. In:
Culture of Marine Invertebrate Animals. W. L. Smith and M. H. Changley, eds., Plenus
Publishing Corp., New York
Hasan, J. A., Huq, A., Tamplin, M. L., Siebeling, R. J. and R.R. Colwell. (1994). A novel kit for
rapid detection of
Vibrio cholerae
O1.
J Clin Microbiol
. 32: 249–252.
Miller, A.W., M. Frazier, G.E. Smith, E.S. Perry, G.M. Ruiz, and M.N. Tamburri, 2011.
Enumerating Sparse Organisms in Ships’ Ballast Water: Why Counting to 10 is Difficult?
Environ. Sci. Tech.
, 45:3539-3546.
Steinberg, M.K., E.J. Lemieux, and L.A. Drake, 2011. Determining the viability of marine
protists using a combination of vital, fluorescent stains.
Mar Biol
158:1431-1437.