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Appendix D
5/20/2009
12
11.2.2.2 Add 40 ml of 90% acetone, shake well, and place in a
dark box in the refrigerator for 2-24 hours. Overnight is
recommended.
11.2.2.3 Follow procedure for sediments after extraction. Dilute
the sample as needed.
11.3 Pollution Prevention and Waste Management
11.3.1 This method generates hazardous waste.
11.3.2 Acetone waste is stored in 4 liter jugs in the cabinet under
the hood and transferred to the hazardous waste area of the
Storage Facility on campus.
11.3.3 Do not pour acetone down the sink.
11.3.4 Decant the waste acetone into the waste jugs, and then
allow the remaining ground filter pad or sediment to dry in
the hood.
11.3.5 The dried waste may then be put in the trash.
11.4 How to run the Turner Designs TD700
11.4.1 The TD700 is set up to utilize the acidification method,
with the filters located in B on the filter holder. If the non-
acid method is to be used, the filters are located in A on the
filter holder, which must then be moved to that orientation
and calibrated.
11.4.1
Turn on power and allow fluorometer to warm up for a
minimum of 1 hour.
11.4.2
Take samples out of the refrigerator and allow them to
come to room temperature.
11.4.3
Centrifuge samples for 5-10 minutes at ~2300 rpm.
11.4.4
Fill the cuvette at least 2/3 full (approx. 5-6 ml) with 90%
acetone. Wipe the cuvette with lens paper to clean and
insert the cuvette with acetone blank into sample well.
11.4.5
Press the “*” key on the keypad. This is the “discrete
averaging” function allowing all samples to be analyzed for
the same amount of time. Take the reading when the
instrument reports END.
11.4.6
Remove the acetone blank and the cuvette adaptor. Place
the solid secondary standard within the sample well. Press
“*”. Read both the low and the high sides. Compare the
readings to the original solid standard reading during
calibration. If the high std is off by 2.5% or more, reset the
calibration to the high solid standard reading acquired at
calibration, using the calibration function.
11.4.7 Samples may now be analyzed.
11.4.8 Optical Kit: extractive acidification method
11.4.8.1
Daylight White Lamp PN 10-045
11.4.8.2
Excitation filter 340-500 nm PN 10-050R