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Appendix D
5/20/2009
13
11.4.8.3
Emission filter >665 nm PN 10-051R
11.4.9 Optical Kit: non-acid method
11.4.8.1
Blue mercury Vapor Lamp PN 10-089
11.4.8.2
Excitation filter 436 nm PN 10-113
11.4.8.3
Emission filter 680 nm PN 10-115
11.5 How to operate the Turner Designs Trilogy
11.5.1 Connect the Trilogy to the desktop computer by the serial
port cable(s).
11.5.2 Open the SIS for Trilogy software on the desktop. Click
START. An EXCEL spreadsheet will open. Minimize the
SIS file.
11.5.3 Turn on the instrument. The instrument has a touch screen.
The non-acid optical module is in place. Touch the CHL-
NA button to confirm this. (There is also available the
acidification optical module (CHL-A). Change the
modules to analyze samples by the acidification method.
The Trilogy must be recalibrated each time an optical
module is changed. )
11.5.4 The Trilogy does not need a long warm up period.
11.5.5 Confirm that the instrument and the computer are talking to
each other. A line of gibberish and Turner Designs will
appear in the spreadsheet.
11.5.6 Press the CALIBRATE button to select a standard curve,
new or existing. Up to 5 standards may be used. Once
selected, press View Curve, and then select it to record in
the spreadsheet.
11.5.7 Pipette the samples into the 2 ml screw-cap vials, and cap.
Wipe the vial with lens paper to insure it is dry and has no
smudges. Enter the sample name by pressing the sample
ID button on the upper left side of the screen.
11.5.8 A solid secondary standard is available as a check on the
calibration. Take out the vial adaptor to insert the solid
standard.
11.5.9 To read direct concentration, press the MEASURE
FLUORESCENCE button. Enter the volume filtered and
press OK. (For standards and blanks enter 1.) Enter the
extract volume. (For standards and blanks enter 1.) The
instrument will then confirm the choices and begin the
analysis. This is the direct concentration mode, so only
dilutions need to be factored into the extract volume.
11.5.10 When all samples are run, SAVE the file to the desktop in
the Trilogy chla folder. Do not close the SIS software until
the EXCEL spreadsheet has been saved.
11.5.11 It is now safe to close the SIS software. It will also close
the spreadsheet.
11.5.12 Turn off the instrument.