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SOP2 – Challenge Water Modifications
20
Chlamydomonas_cf
sp.
(12-16 µm in minimum dimension). Other possible candidates include
the following native phytoplankton:
Chaetoceros gracile
(10-15 µm in minimum dimension),
Asterionellopsis glacialis (20-25 µm in minimum dimension) and
Coscinodiscus radiates
(12-16
µm in minimum dimension).
3.3.3. Plankton Injection System Materials
•
1.1 m
3
(300 gallon) 45-degree cone-bottomed plastic tank with lid, marked at 1m
3
•
Air system to homogenize the mixture
•
Eccentric peristaltic pump SLP-218 with Lenze AC Tech VFD
•
Extra peristaltic pump interior hose, hose clamps and oil
•
Delivery hose (from slurry pump to pump-skid intake valve)
•
Face masks
,
gloves
,
goggles
•
Plankton cultures as needed
•
Ambient water source
3.3.4. Plankton Mixing/Injection Procedures
Summary
•
Fill mixing tank to 1
m
3
.
•
Add concentrated mix of plankton.
•
Engage aeration system.
•
Add the plankton slurry to the ambient water flow stream on a time-average basis using
an industrial peristaltic pump. The pump flow is finely controlled using a variable-
frequency drive (VFD).
Details
•
Attach the delivery hose from the peristaltic pump to the pump skid hose.
•
Fill the mixing tank to the 1 m
3
mark with water.
•
Turn on the aeration system.
•
Slowly add the plankton into the top port.
•
Post one operator at the pump skid intake valve and one at the plankton (peristaltic) pump
VFD.
•
Turn on the plankton pump VFD, increasing the speed to 10-20 Hz while opening the
delivery hose intake valve on the skid.
•
Slowly ramp up the plankton pump speed to the hertz required to meet the challenge
water requirements.
•
The plankton pump operator monitors the plankton pump and tank levels, relaying
information to the skid operator.
•
After ballast tanks are filled and when the ballast skid operator directs, turn off plankton
pump while closing the skid intake valve. Disconnect delivery hose from the skid and
drain remaining plankton from tank.
Freshwater flush all systems as directed in section
3.2.5.