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SOP2 – Challenge Water Modifications
19
3.2.8. 2012 Slurry Injection Data Sheet
3.3. Increasing Live Organism Abundances to Meet Challenge Water Requirements
The liquid mixture created to enhance the ambient water biological conditions to meet ETV
protocols/G8 guidelines is defined as “biological slurry”. Specific species and concentrations
added are determined according to the ambient water concentrations, pump-skid uptake flow
rates, uptake volumes and Test Plan requirements.
3.3.1. Live Organisms > 50 microns
During the testing season (March – November), ambient Chesapeake Bay organisms > 50
µ
m
commonly meet or exceed the challenge concentration of 100,000 live organisms per m
3
listed in
the G8 guidelines and ETV protocols. However, if necessary, to increase challenge water
concentrations of zooplankton, larvae (> 100
µ
m in size) of the native eastern oyster
(
Crassostrea virginica
) are added. Larvae are obtained from the UMCES Horn Point Laboratory
Oyster Hatchery (www.hpl.umces.edu/hatchery) less than 24 hours prior to use in a test trial.
3.3.2. Live Organisms Between 10 – 50 microns - Augmentation
During the testing season (March – November), ambient Chesapeake Bay organisms 10 – 50
µ
m
in size commonly meet or exceed the challenge concentration of 1,000 live organisms per ml
listed in the G8 guidelines and ETV protocols. However, if necessary, to
increase challenge
water concentrations of phytoplankton, algae cultured at the University of Maryland Wye
Research and Education Center are added to challenge water. The genus used will generally be
Evals>2010>MSI
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7/11/12
2012 Slurry Data Sheet
MERC Barge
Vendor/Trial Number
Tech QAQC Copy Scan
Date
Water Used
Slurry recipe
____ lbs Micromate (20?)
_____ total jugs AZ test dust __x__Fine (3.5 kg/ 7.7 lbs each) 8.5 = TSS-50, 4 = TSS 25)
____ jars tea (3 oz. or 0.19 lbs each) (70?)
Tide :
Target Pump Skid Flow Rate (Hz and L/Min):
(40 hz = 11.5 L/min)
Target Ballast Tank Volume
Sample Port Location
Challenge - pre vendor
Other Notes:
Sample Sample
Tank Slurry Pipe TSS TSS
PC
PC
DOC DOC
%UVT %UVT
Start
Vol
Pump Flow mg/L mg/L mg/L mg/L mg/L mg/L 254 254
ID
Time
m3
Hz m3/hr Control
Chall-
enge Control
Challe-
nge Control
Chall-
enge Control
Chall-
enge
AMB-Ini
0
0
CH-1
CH-2
CH-3
WW-C
WW-T
Amb Final
NA
CH = Challenge Water
WW = whole water or time integrated sample